Events

Single-molecule studies on structure, dynamics and interactions of proteins: mechanisms and tool development

Event date: 
28 February 2020 - 11:00am to 12:00pm
Location: 
Kirby Seminar Room, Level 6, Wallace Wurth Building UNSW
Event Type: 
Seminar
File Attachment: 
Booking deadline: 

The Cell Motility & Mechanobiology group and SoMS are proud to host Prof. Jörg Fitter for a special seminar.

Abstract

Cell-free protein synthesis systems offer enormous opportunities for the
application of single-molecule techniques. For example, by employing confocal two-color
coincidence detection (TCCD) the fraction of active ribosomes in a population, as well as their
productivity can be determined [1]. Based on a further development of the TCCD method, we
monitored the subunit dissociation of 70S ribosomes for translation initiation in a cell free
transcription/translation assay and unraveled a previously undetermined second initiation
pathway in bacterial protein synthesis [2]. In a further approach we extended single-molecule
FRET (smFRET) studies, which typically require bright organic fluorescent dyes, to genetically
encoded FRET-based biosensors with two different fluorescent proteins employed as donor/
acceptor pair. The obtained smFRET data provides valuable insights into the sensor
performance and thereby helps to understand and to optimise the design of FRET-based
sensors [3]. In a last example we investigated unfolding/folding transitions in a multi-domain
protein. For phosphyglycerate kinase, a two-domain protein, we mapped several intra-molecular
distances upon chemical denaturation to identify folding intermediates [4]. A key in producing
suitable FRET samples for multiple distances is to make use of new fluorescent labelling
strategies, for example by employing unnatural amino acids in cell-free protein synthesis [5].

References

1. N. Kempf et al., Scientific Reports, 7: 46753, (2017)
2. H. Höfig et al., Communications Biology, 2: 459, (2019)
3. H. Höfig et al., ACS Sensors, 3: 1462-1470, (2018)
4. M. Cerminara et al., Biophysical Journal, in press (2020)
5. M. Sadoine et al., Analytical Chemistry, 89: 11278-11285, (2017)

For information or to arrange a meeting with the speaker contact 
Daryan Kempe: d.kempe@unsw.edu.au or Maté Biro: m.biro@unsw.edu.au

Image - Single-molecule studies on structure, dynamics and interactions of proteins: mechanisms and tool development

Calendar of Events

Su Mo Tu We Th Fr Sa
 
 
 
 
 
 
1
 
2
 
3
 
4
 
5
 
6
 
7
 
8
 
9
 
10
 
11
 
12
 
13
 
14
 
15
 
16
 
17
 
18
 
19
 
20
 
21
 
22
 
23
 
24
 
25
 
26
 
27
 
28
 
29
 

21 February 2020

No events found

Add your event

Submit your event to UNSW events. Only events run by UNSW will be accepted. Once approved your event will appear on both the UNSW and Medicine websites.

Add to UNSW Events

 

If your event has a local focus, submit your event to Medicine events. Only events run by Medicine will be accepted. Your event will only appear on Medicine websites. Do not use this if you have added your event to UNSW events.

Add to Medicine Events